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anti phospho prb s780 polyclonal  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti phospho prb s780 polyclonal
    Anti Phospho Prb S780 Polyclonal, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 869 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+anti+phospho-prb+(s780)/Phospho-Rb+(Ser780)+Antibody/pm27214403-470-31-44
    Average 96 stars, based on 869 article reviews
    anti phospho prb s780 polyclonal - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: The transcription factor MEF2C restrains microglial overactivation by inhibiting kinase CDK2.
    Article Snippet: In brief MEF2C is an essential immune checkpoint that prevents microglial overactivation, though its mechanisms remain elusive.. Hu et al. reveal that MEF2C restrains microglial overactivation via modulating the p21CDK2-RB-NFkB axis.. In Mef2c-deficient mice, the CDK2 inhibitor BMS265246 mitigates microglial overactivation, neuronal loss, and ASD-like behaviors, establishing CDK2 as a promising therapeutic target for neurological disorders associated with microglial overactivation.

    Article Title: IRF1 Mediates Growth Arrest and the Induction of a Secretory Phenotype in Alveolar Epithelial Cells in Response to Inflammatory Cytokines IFNγ/TNFα
    Article Snippet: 20 μg of proteins were separated on BoltTM 4–12% Bis-Tris mini protein gel (Thermo Fisher Scientific) and transferred to PVDF membranes (Immobilon-P membrane, Thermo Fisher Scientific). .. Membranes were incubated for 1 h at room temperature (RT) in blocking solution (4% non-fat dried milk in TBST, Tris-buffered saline solution + 0.5% Tween), then overnight at 4 °C with anti-IRF1, anti-p21 WAF , anti-pRB-S780, anti-pSTAT1-Tyr701, or anti-ICAM-1 rabbit polyclonal antibody or anti-cyclin D3 monoclonal antibody (1:2000, Cell Signaling Technology, Euroclone, Pero (MI), Italy) in TBST containing 5% BSA. .. Anti-vinculin mouse monoclonal antibody (1:2000, Merck) was used as a loading control.

    Western Blot:

    Article Title: MUC1-C dependency in drug resistant HR+/HER2- breast cancer identifies a new target for antibody-drug conjugate treatment.
    Article Snippet: Total lysates and chromatin-bound protein prepared from nonconfluent cells were subjected to immunoblot analysis using antiMUC1-C (#MA5–11202, 1:1000 dilution; Thermo Fisher Scientific, Waltham, MA, USA), anti-NaKATPase1 (3010S, 1:1000, Cell Signaling Technology, Danvers, MA, USA), anti-LaminB1 (66095-1-Ig, 1:2000, Proteintech, Rosemont, IL, USA), anti-α-tubulin (2144S, 1:1000, CST), anti-H3 (9715S, 1:1000, CST), anti-ER (8644S, 1:1000, Cell Signaling Technology), anti-β-actin (A5441, 1:10,000 dilution, Sigma-Aldrich), anti-SRC-3 (2126S, 1:1000, CST), anti-pSRC3(S857) (PA5-106189, Thermo Fischer Scientific), anti-MK2 (12155S, 1:1000, CST), antiMED1 (51613S, 1:1000, CST), anti-pMED1(T1457) (ab60950, 1:500, abcam), anti-CDK7 (2916S, 1:1000, CST), anti—pCDK7(T170) (ab155976, 1:1000, abcam), anti-pPol II(pRpb1)(S7) (13780S, 1:500, CST), anti-Pol II(Rpb1) (14958T, 1:1000, CST), anti-pRB(S780) (9307S, 1:1000, CST), anti-RB (9309S, 1:1000, CST) and anti-E2F1 (3742, 1:1000, CST) as described25. .. Total lysates and chromatin-bound protein prepared from nonconfluent cells were subjected to immunoblot analysis using antiMUC1-C (#MA5–11202, 1:1000 dilution; Thermo Fisher Scientific, Waltham, MA, USA), anti-NaKATPase1 (3010S, 1:1000, Cell Signaling Technology, Danvers, MA, USA), anti-LaminB1 (66095-1-Ig, 1:2000, Proteintech, Rosemont, IL, USA), anti-α-tubulin (2144S, 1:1000, CST), anti-H3 (9715S, 1:1000, CST), anti-ER (8644S, 1:1000, Cell Signaling Technology), anti-β-actin (A5441, 1:10,000 dilution, Sigma-Aldrich), anti-SRC-3 (2126S, 1:1000, CST), anti-pSRC3(S857) (PA5-106189, Thermo Fischer Scientific), anti-MK2 (12155S, 1:1000, CST), antiMED1 (51613S, 1:1000, CST), anti-pMED1(T1457) (ab60950, 1:500, abcam), anti-CDK7 (2916S, 1:1000, CST), anti—pCDK7(T170) (ab155976, 1:1000, abcam), anti-pPol II(pRpb1)(S7) (13780S, 1:500, CST), anti-Pol II(Rpb1) (14958T, 1:1000, CST), anti-pRB(S780) (9307S, 1:1000, CST), anti-RB (9309S, 1:1000, CST) and anti-E2F1 (3742, 1:1000, CST) as described25. .. Nuclear proteins were incubated with anti-MUC1-C (#MA5–11202; Thermo Fisher Scientific), antiSRC-3 (2126S, CST), anti-MED1 (51613S, CST), Armenian Hamster IgG (ab18479, abcam) or Rabbit IgG (#NI01-100UG, Sigma), precipitated with Dynabeads Protein G (#10004D; Thermo Fisher Scientific) and analyzed as described57.

    Article Title: MUC1-C dependency in drug resistant HR+/HER2− breast cancer identifies a new target for antibody-drug conjugate treatment
    Article Snippet: .. Total lysates and chromatin-bound protein prepared from non-confluent cells were subjected to immunoblot analysis using anti-MUC1-C (#MA5–11202, 1:1000 dilution; Thermo Fisher Scientific, Waltham, MA, USA), anti-NaKATPase1 (3010S, 1:1000, Cell Signaling Technology, Danvers, MA, USA), anti-LaminB1 (66095-1-Ig, 1:2000, Proteintech, Rosemont, IL, USA), anti-α-tubulin (2144S, 1:1000, CST), anti-H3 (9715S, 1:1000, CST), anti-ER (8644S, 1:1000, Cell Signaling Technology), anti-β-actin (A5441, 1:10,000 dilution, Sigma-Aldrich), anti-SRC-3 (2126S, 1:1000, CST), anti-pSRC3(S857) (PA5-106189, Thermo Fischer Scientific), anti-MK2 (12155S, 1:1000, CST), anti-MED1 (51613S, 1:1000, CST), anti-pMED1(T1457) (ab60950, 1:500, abcam), anti-CDK7 (2916S, 1:1000, CST), anti—pCDK7(T170) (ab155976, 1:1000, abcam), anti-pPol II(pRpb1)(S7) (13780S, 1:500, CST), anti-Pol II(Rpb1) (14958T, 1:1000, CST), anti-pRB(S780) (9307S, 1:1000, CST), anti-RB (9309S, 1:1000, CST) and anti-E2F1 (3742, 1:1000, CST) as described . ..

    other:

    Article Title: A novel telomerase-derived peptide GV1001-mediated inhibition of angiogenesis: Regulation of VEGF/VEGFR-2 signaling pathways
    Article Snippet: The following agents were obtained from commercial sources: vascular endothelial growth factor-A 165 (Merck Millipore, Billerica, MA, USA); anti-phospho-VEGFR-2 (Y1175), anti-phospho-MEK (S217/S221), anti-MEK, anti-phospho-Src (Y416), anti-Src, anti-phospho-p70 S6K (T421/S424), anti-phospho-Akt (S473), anti-phospho-ERK (T202/Y204), anti-phospho-pRb (S780), and anti-phospho-pRb (S807/S811) (Cell Signaling Technology, Beverly, MA, USA); fibroblast growth factor-2 (FGF-2), anti-phosphotyrosine, anti-phospho-FAK(Y397), anti-FAK, anti-β-catenin, and anti-p120-catenin (BD Biosciences, Bedford, MA, USA); anti-vascular endothelial (VE)-cadherin, anti-VEGFR-2, anti-p70 S6K , anti-Akt, anti-ERK, anti-Cdk2, anti-Cdk4, anti-cyclin D, anti-cyclin E, anti-p27 kip1 , anti-actin antibodies, and mouse and rabbit IgG-horseradish peroxidase conjugates (Santa Cruz Biotechnology, Santa Cruz, CA, USA); goat anti-mouse IgG-Alexa Fluor 488 conjugate (Thermo Fisher Scientific Co., Waltham, MA, USA).

    CRISPR:

    Article Title: Resistance mechanism to Notch inhibition and combination therapy in human T-cell acute lymphoblastic leukemia
    Article Snippet: .. Target Company Clone # Catalog # RRID # pAKT(T308) Cell Signaling D25E6 13038s AB_2629447 pAKT(S473) Cell Signaling D9E 4060s AB_2315049 pAKT(T450) Cell Signaling polyclonal 9267s AB_823676 AKT Cell Signaling polyclonal 9272s AB_329827 NICD (V1744) Cell Signaling D3B8 4147s AB_2153348 C-MYC Abcam Y69 ab32072 AB_731658 P85 Cell Signaling 19H8 4257 AB_659889 pS6K (T421/S424) Cell Signaling polyclonal 9204s AB_2265913 pS6K (T389) Cell Signaling polyclonal 9205s AB_330944 p70 S6K Cell Signaling polyclonal 9202s AB_331676 pS6 Ribosomal protein (S235/236) Cell Signaling polyclonal 2211s AB_331679 S6 Ribosomal protein Cell Signaling 54D2 2317s AB_2238583 CRISPR-CAS9 Abcam 7A9-3A3 ab191468 AB_2692325 BCL-xL Abcam polyclonal ab98143 AB_10674728 BCL2 Cell Signaling polyclonal 2872T AB_10693462 TATA binding proteinTBP Abcam mAbcam51841 ab51841 AB_945758 p-Tuberin/TSC2 (S939) Cell Signaling polyclonal 3615T AB_2207796 Tuberin/TSC2 Cell Signaling D93F12 4308T AB_10547134 pBAD (S136) Cell Signaling D25H8 4366T AB_10547878 BAD Cell Signaling D24A9 9239T AB_2062127 pRB (S807/811) Cell Signaling D20B12 8516S AB_11178658 pRB (S780) Cell Signaling polyclonal 9307T AB_330015 pRB (S795) Cell Signaling unknown 9301T AB_330013 RB Abcam EPR17512 ab181616 AB_2848193 ACTIN Abcam 8226 ab8226 AB_306371 anti-mouse IgG, HPR-linked Cell Signaling polyclonal 7076S AB_330924 anti-rabbit IgG, HPR-linked Cell Signaling polyclonal 7074S AB_2099233 .. Name Target CAS Provider Catalog # Delivery Palbociclib CDK4/6:CCND3 827022-32-2 Chemietek CT-PD2991 Oral Gavage Ruxolitinib JAK1/2 941678-49-5 Selleck Chemicals S1378 in vitro Venetoclax BCL2 1257044-40-8 LuBioScience HY-15531 Oral Gavage MK-2206 AKT 1032349-77-1 LuBioScience HY-10358 Oral Gavage Idelalisib PI3Kdelta 870281-82-6 LuBioScience S2226 in vitro CB-103 Notch 218457-67-1 Chemie Brunschwig AG CBLSS-5069 intraperitoneal LY3039478 Notch 1421438-81-4 SpiroChem S7169 intraperitoneal DAPT Notch 208255-80-5 Selleck Chemicals S2215 in vitro

    Binding Assay:

    Article Title: Resistance mechanism to Notch inhibition and combination therapy in human T-cell acute lymphoblastic leukemia
    Article Snippet: .. Target Company Clone # Catalog # RRID # pAKT(T308) Cell Signaling D25E6 13038s AB_2629447 pAKT(S473) Cell Signaling D9E 4060s AB_2315049 pAKT(T450) Cell Signaling polyclonal 9267s AB_823676 AKT Cell Signaling polyclonal 9272s AB_329827 NICD (V1744) Cell Signaling D3B8 4147s AB_2153348 C-MYC Abcam Y69 ab32072 AB_731658 P85 Cell Signaling 19H8 4257 AB_659889 pS6K (T421/S424) Cell Signaling polyclonal 9204s AB_2265913 pS6K (T389) Cell Signaling polyclonal 9205s AB_330944 p70 S6K Cell Signaling polyclonal 9202s AB_331676 pS6 Ribosomal protein (S235/236) Cell Signaling polyclonal 2211s AB_331679 S6 Ribosomal protein Cell Signaling 54D2 2317s AB_2238583 CRISPR-CAS9 Abcam 7A9-3A3 ab191468 AB_2692325 BCL-xL Abcam polyclonal ab98143 AB_10674728 BCL2 Cell Signaling polyclonal 2872T AB_10693462 TATA binding proteinTBP Abcam mAbcam51841 ab51841 AB_945758 p-Tuberin/TSC2 (S939) Cell Signaling polyclonal 3615T AB_2207796 Tuberin/TSC2 Cell Signaling D93F12 4308T AB_10547134 pBAD (S136) Cell Signaling D25H8 4366T AB_10547878 BAD Cell Signaling D24A9 9239T AB_2062127 pRB (S807/811) Cell Signaling D20B12 8516S AB_11178658 pRB (S780) Cell Signaling polyclonal 9307T AB_330015 pRB (S795) Cell Signaling unknown 9301T AB_330013 RB Abcam EPR17512 ab181616 AB_2848193 ACTIN Abcam 8226 ab8226 AB_306371 anti-mouse IgG, HPR-linked Cell Signaling polyclonal 7076S AB_330924 anti-rabbit IgG, HPR-linked Cell Signaling polyclonal 7074S AB_2099233 .. Name Target CAS Provider Catalog # Delivery Palbociclib CDK4/6:CCND3 827022-32-2 Chemietek CT-PD2991 Oral Gavage Ruxolitinib JAK1/2 941678-49-5 Selleck Chemicals S1378 in vitro Venetoclax BCL2 1257044-40-8 LuBioScience HY-15531 Oral Gavage MK-2206 AKT 1032349-77-1 LuBioScience HY-10358 Oral Gavage Idelalisib PI3Kdelta 870281-82-6 LuBioScience S2226 in vitro CB-103 Notch 218457-67-1 Chemie Brunschwig AG CBLSS-5069 intraperitoneal LY3039478 Notch 1421438-81-4 SpiroChem S7169 intraperitoneal DAPT Notch 208255-80-5 Selleck Chemicals S2215 in vitro

    Blocking Assay:

    Article Title: IRF1 Mediates Growth Arrest and the Induction of a Secretory Phenotype in Alveolar Epithelial Cells in Response to Inflammatory Cytokines IFNγ/TNFα
    Article Snippet: 20 μg of proteins were separated on BoltTM 4–12% Bis-Tris mini protein gel (Thermo Fisher Scientific) and transferred to PVDF membranes (Immobilon-P membrane, Thermo Fisher Scientific). .. Membranes were incubated for 1 h at room temperature (RT) in blocking solution (4% non-fat dried milk in TBST, Tris-buffered saline solution + 0.5% Tween), then overnight at 4 °C with anti-IRF1, anti-p21 WAF , anti-pRB-S780, anti-pSTAT1-Tyr701, or anti-ICAM-1 rabbit polyclonal antibody or anti-cyclin D3 monoclonal antibody (1:2000, Cell Signaling Technology, Euroclone, Pero (MI), Italy) in TBST containing 5% BSA. .. Anti-vinculin mouse monoclonal antibody (1:2000, Merck) was used as a loading control.

    Saline:

    Article Title: IRF1 Mediates Growth Arrest and the Induction of a Secretory Phenotype in Alveolar Epithelial Cells in Response to Inflammatory Cytokines IFNγ/TNFα
    Article Snippet: 20 μg of proteins were separated on BoltTM 4–12% Bis-Tris mini protein gel (Thermo Fisher Scientific) and transferred to PVDF membranes (Immobilon-P membrane, Thermo Fisher Scientific). .. Membranes were incubated for 1 h at room temperature (RT) in blocking solution (4% non-fat dried milk in TBST, Tris-buffered saline solution + 0.5% Tween), then overnight at 4 °C with anti-IRF1, anti-p21 WAF , anti-pRB-S780, anti-pSTAT1-Tyr701, or anti-ICAM-1 rabbit polyclonal antibody or anti-cyclin D3 monoclonal antibody (1:2000, Cell Signaling Technology, Euroclone, Pero (MI), Italy) in TBST containing 5% BSA. .. Anti-vinculin mouse monoclonal antibody (1:2000, Merck) was used as a loading control.



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    Cell Signaling Technology Inc anti phospho prb s780 polyclonal
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